Bruker Avance III UltraStabilized 800 MHz Spectrometer
This system is housed in Schweitzer Hall. It has the latest and most advanced Ultra-Shield-Plus magnet with four radiofrequency (rf) channels, an ATM (automatic tuning and matching) and a TCI (1H/13C/15N) probe with cryo-chilled 1H and 13C preamplifiers.
Figure 6 (Below). Bicelles Alter Auto-inhibitory Conformation and Stimulate Activation
This research was originally published in Structure. Stephen H. Prior, Yan G. Fulcher, Rama K. Koppisetti, Alexander Jurkevich, Steven R. Van Doren. Charge-Triggered Membrane Insertion of Matrix Metalloproteinase-7, Supporter of Innate Immunity and Tumors. Nature Communications. October 1, 2015; Volume 23, Issue 11, 3 November 2015, Pages 2099–2110. © Cell Press, Elsevier Inc. http://www.sciencedirect.com/science/article/pii/S0969212615003639
Figure 2 (Below): PRE-NMR structural ensembles and interfacial contacts of DMPC bilayer bound to opposite sides of catalytic domain.
This research was originally published in Nature Communications. Rama K. Koppisetti, Yan G. Fulcher, Alexander Jurkevich, Stephen H. Prior, Jia Xu et al. Ambidextrous binding of cell and membrane bilayers by soluble matrix metalloproteinase-12. Nature Communications. Nov 21, 2014; 5, Article number: 5552 (2014) doi:10.1038/ncomms6552. © Nature Publishing Group. https://www.nature.com/articles/ncomms6552
Fig. 7 (Below) - Productive complex of a collagen triple helix in the active site of an MMP revealed by paramagnetic NMR.
This research was originally published in Journal of Biological Chemistry (JBC). Stephen H. Prior, Todd S. Byrne, Dorota Tokmina-Roszyk, Gregg B. Fields, and Steven R. Van Doren. Path to Collagenolysis COLLAGEN V TRIPLE-HELIX MODEL BOUND PRODUCTIVELY AND IN ENCOUNTERS BY MATRIX METALLOPROTEINASE-12. Journal of Biological Chemistry (JBC). 2016; Vol: 291, p7888-7901. © the American Society for Biochemistry and Molecular Biology. http://www.jbc.org/content/291/15/7888.long
Fig 1. (Below) Comparison of loading and score plots generated from preprocessed NMR spectra of EBC from 53 cats before (black) and after induction of early allergic asthma (red), using multivariate statistics. Panels A and D show PCA results. Panels B and E plot PLS-DA results. Panels C and F report multi-level PLS-DA results. Components 1 and 2 are plotted in the loading plots (A–C). Components 1, 2, and 3 are plotted in the score plots (D–F). Biomarkers that increased in the experimental asthma are labeled in red. A marker that decreased in the experimental asthma is labeled in blue. A peak too weak to be useful is labeled in gray.
Citation: Fulcher YG, Fotso M, Chang C-H, Rindt H, Reinero CR, Van Doren SR (2016) Noninvasive Recognition and Biomarkers of Early Allergic Asthma in Cats Using Multivariate Statistical Analysis of NMR Spectra of Exhaled Breath Condensate. PLoS ONE 11(10): e0164394. https://doi.org/10.1371/journal.pone.0164394
Please contact Dr. Shaokai Jiang for training and questions about analyses.